提供覆蓋分子生物學(xué)、細(xì)胞生物學(xué)、植物學(xué)、生物化學(xué)、蛋白組學(xué)、免疫學(xué)等領(lǐng)域的實(shí)驗(yàn)產(chǎn)品以及生物技術(shù)服務(wù)等
普通會(huì)員
產(chǎn)品價(jià)格¥450.00元/mg
產(chǎn)品品牌MCE
最小起訂≥5 mg
供貨總量100000 mg
發(fā)貨期限自買(mǎi)家付款之日起 3 天內(nèi)發(fā)貨
瀏覽次數(shù)87
企業(yè)旺鋪http://qdjcys.com/gongsi/qq396945203/
更新日期2022-08-26 15:15
會(huì)員級(jí)別:企業(yè)會(huì)員
身份認(rèn)證:
已 繳 納:0.00 元保證金
我的勛章: [誠(chéng)信檔案]
企業(yè)二維碼: 企業(yè)名稱加二維碼
聯(lián) 系 人:鄧(女士)
電子郵箱:[email protected]
聯(lián)系手機(jī):15906629305
聯(lián)系固話:15906629305
聯(lián)系地址:江干區(qū)天城路
品牌: |
MCE |
所在地: |
浙江 杭州市 |
起訂: |
≥5 mg |
供貨總量: |
100000 mg |
有效期至: |
長(zhǎng)期有效 |
Puromycin dihydrochloride
Puromycin dihydrochloride (CL13900 dihydrochloride) 是一種氨基核苷類抗生素,抑制蛋白合成 (protein synthesis)。
體外研究(In Vitro)
Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1].
Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2].
When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting
分子量:544.43
Formula:C22H31Cl2N7O5
CAS 號(hào):58-58-2
來(lái)源:Streptomyces alboniger
運(yùn)輸條件:Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式:
4°C, sealed storage, away from moisture
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
參考文獻(xiàn)
[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92.
[2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 2000 Mar 1;28(5):1176-82.
[3]. Schmidt EK, et al. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods. 2009 Apr;6(4):275-7.
還沒(méi)找到您需要的生化試劑產(chǎn)品?立即發(fā)布您的求購(gòu)意向,讓生化試劑公司主動(dòng)與您聯(lián)系!
立即發(fā)布求購(gòu)意向本網(wǎng)頁(yè)所展示的有關(guān)【Puromycin dihydrochloride_生化試劑_杭州昊鑫生物科技股份有限公司】的信息/圖片/參數(shù)等由商易網(wǎng)的會(huì)員【杭州昊鑫生物科技股份有限公司】提供,由商易網(wǎng)會(huì)員【杭州昊鑫生物科技股份有限公司】自行對(duì)信息/圖片/參數(shù)等的真實(shí)性、準(zhǔn)確性和合法性負(fù)責(zé),本平臺(tái)(本網(wǎng)站)僅提供展示服務(wù),請(qǐng)謹(jǐn)慎交易,因交易而產(chǎn)生的法律關(guān)系及法律糾紛由您自行協(xié)商解決,本平臺(tái)(本網(wǎng)站)對(duì)此不承擔(dān)任何責(zé)任。您在本網(wǎng)頁(yè)可以瀏覽【Puromycin dihydrochloride_生化試劑_杭州昊鑫生物科技股份有限公司】有關(guān)的信息/圖片/價(jià)格等及提供【Puromycin dihydrochloride_生化試劑_杭州昊鑫生物科技股份有限公司】的商家公司簡(jiǎn)介、聯(lián)系方式等信息。
在您的合法權(quán)益受到侵害時(shí),歡迎您向郵箱發(fā)送郵件,或者進(jìn)入《網(wǎng)站意見(jiàn)反饋》了解投訴處理流程,我們將竭誠(chéng)為您服務(wù),感謝您對(duì)商易網(wǎng)的關(guān)注與支持!