1. 產(chǎn)品參數(shù):
Ex(nm) 562 Em(nm) 576
分 子 量 1015.79 溶劑 DMSO
存儲(chǔ)條件 在零下15度以下保存,避免光照
2. 產(chǎn)品概述:
鈣黃綠素紅,AM是美國(guó)AAT Bioquest生產(chǎn)的用于檢測(cè)活細(xì)胞的熒光探針,Calcein AM是用于標(biāo)記和檢測(cè)活細(xì)胞細(xì)胞功能的流行的熒光探針之一。然而,Calcein AM的單色使得在多色應(yīng)用中不可能使用這種有價(jià)值的試劑。例如,由于與GFP相同的顏色,不可能將Calcein AM與GFP-轉(zhuǎn)化細(xì)胞組合使用。為了解決Calcein AM的這種顏色限制,我們開(kāi)發(fā)了Calcein Orange ,Calcein Red 和Calcein Deep Red 。這些新的Calcein AM類似物與Calcein AM結(jié)合使用,可以對(duì)活細(xì)胞進(jìn)行多色標(biāo)記和功能分析。非熒光Calcein Red AM可以輕松進(jìn)入活細(xì)胞并水解產(chǎn)生強(qiáng)熒光Calcein Red 染料??梢允褂贸R?jiàn)的TRITC / Cy3濾波片組檢測(cè)Calcein Red 染料。百螢生物是AAT Bioquest的中國(guó)代理商,為您提供優(yōu)質(zhì)的鈣黃綠素紅,AM。
3. 適用儀器:
(1)流式細(xì)胞儀
Ex: 532/561nm
Em: 585/40nm
(2)熒光酶標(biāo)儀
Ex: 540nm
Em: 590nm
Cutoff: 570nm
推薦孔板: 黑色透明底板
讀取模式: 底讀模式
(3)熒光顯微鏡
Ex: TRITC濾波片組
Em: TRITC濾波片組
推薦孔板: 黑色透明底板
4. 參考文獻(xiàn)
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Authors: Nienke R Wevers, Dhanesh G Kasi, Taylor Gray, Karlijn J Wilschut, Benjamin Smith, Remko van Vught, Fumitaka Shimizu, Yasuteru Sano, Takashi Kanda, Graham Marsh
Journal: Fluids and Barriers of the CNS (2018): 23
Astragaloside IV protects blood-brain barrier integrity from LPS-induced disruption via activating Nrf2 antioxidant signaling pathway in mice
Authors: Hongli Li, Ping Wang, Fei Huang, Jinmei Jin, Hui Wu, Beibei Zhang, Zhifei Wang, Hailian Shi, Xiaojun Wu
Journal: Toxicology and applied pharmacology (2017)
Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal: Biomedical Optics Express (2017): 2599--2610
NINJ2--A novel regulator of endothelial inflammation and activation
Authors: Jingjing Wang, Jingjing Fa, Pengyun Wang, Xinzhen Jia, Huixin Peng, Jing Chen, Yifan Wang, Chenhui Wang, Qiuyun Chen, Xin Tu
Journal: Cellular Signalling (2017)
Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Peixi Liu, Yingjie Zhou, Qingzhu An, Yaying Song, Xi Chen, Guo-Yuan Yang, Wei Zhu
Journal: MEDICINE (2016): 1--8
Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Anja Lena Thiebes, Manuel Armin Reddemann, Johannes Palmer, Reinhold Kneer, Stefan Jockenhoevel, Christian Gabriel Cornelissen
Journal: Tissue Engineering Part C: Methods (2016): 322--331
Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez
Journal: Scientific reports (2016)
Inhibition of ABC transport proteins by oil sands process affected water
Authors: Hattan A Alharbi, David MV Saunders, Ahmed Al-Mousa, Jane Alcorn, Alberto S Pereira, Jonathan W Martin, John P Giesy, Steve B Wiseman
Journal: Aquatic Toxicology (2016): 81--88
Rapid generation of collagen-based microtissues to study cell--matrix interactions
Authors: Marie-Elena Brett, Alexandra L Crampton, David K Wood
Journal: Technology (2016): 1--8
Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Journal: Journal of Applied Toxicology (2016)